Separation and Enrichment of Flavonoids in Euonymus Alatus by Solvent Floatation
摘要:
Flavonoids in Euonymus alatus were separated and enriched by solvent floatation,and the amounts of total flavonoids and quercetin were determined by UV-VIS spectrophotometry and HPLC.Floatation was carried out in a solution of pH 3.0 with n-butanol as floatation solvent and by passing N_2-gas through the solution at the rate of 300 mL·min~(-1) for 50 min.As shown by the results of UV-VIS spectrophotometric analysis,the rate of floatation of total flavonoids in Euonymus alatus attained to values in range of 70.8%to 75.0%.The amount of total flavonoids obtained in the floatation liquor after drying by rorary evaporation was 4.6 times higher than that in the extract of the medicine after rotary evaporation drying.With the help of HPLC and using quercetin as the objective analyte,it was found that the floatation rate of quercetin attained to values in the range of 88.9%to 92.2%,and the amount of quercetin in the floatation liquor after rotary evaporation drying was 5.7 times higher than that in the extract of medicine after drying in the same way.
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DOI:
10.1360/972010-874
被引量:
年份:
2010
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