T-cell receptor alpha-chain gene is split in a human T-cell leukemia cell line with a t(11;14)(p15;q11).

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作者：

MML Beau，TW Mckeithan，EA Shima，RE Goldmanleikin，SJ Chan，GI Bell，JD Rowley，MO Diaz

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摘要：

Chromosomal rearrangements in malignant T-cell disease frequently involve the chromosome bands containing the T-cell receptor genes. The RPMI 8402 cell line, which was established from the leukemia cells of a patient with T-cell acute lymphoblastic leukemia, is characterized by a translocation involving chromosome 14 (band q11) and chromosome 11 (band p15) [t(11;14)(p15;q11)]. By using in situ chromosomal hybridization and Southern blot analysis to examine RPMI 8402 cells, we determined that the break at 14q11 occurs within the variable region sequences of the T-cell receptor α -chain gene (TCRA); the break at 11p15 occurs between the HRAS1 gene and the genes for insulin and the insulin-like growth factor 2. These results suggest that the TCRA sequences activate a cellular gene located at 11p15 in malignant T-cell disorders.

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关键词：

Medical Sciences Gene Mapping Lymphoid Neoplasia Chromosomal Translocation Malignant Transformation

DOI：

10.1073/pnas.83.24.9744

被引量：

406

年份：

1986