摘要：

A carbonyl reductase-producing strain Candida sp.PT2A was selected from strain collection of our laboratory and was identified as Candida ontarioensis by 18S rRNA sequencing.The fermentation conditions and bioreduction conditions for the asymmetric synthesis of(R)-2-chloro-1-(3-chlorophenyl)ethanol by Candida ontarioensis were optimized as temperature of 30 ℃,initial pH of 6.5,rotation speed of 180 r/min,and cell concentration of 200 g/L.After for 72 h reaction,the e.e.and the product yield were 99.5% and 99%,respectively on substrate concentration of 10 g/L,whereas the yield was dropped to 84.3% on enhanced substrate concentration of 30 g/L.Using C.ontarioensis whole cells pretreated with 4 g/L CTAB for 20 min at 4 ℃,the product e.e.and the yield reached 99.9% and 97.5% on substrate concentration of 30 g/L at shortened reaction time of 24 h.

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