A novel receptor for Apo2L/TRAIL contains a truncated death domain.

阅读量：

作者：

SA Marsters，JP Sheridan，RM Pitti，A Huang，M Skubatch，D Baldwin，J Yuan，A Gurney，AD Goddard，P Godowski

展开

摘要：

Apo2 ligand (Apo2L [1], also called TRAIL for tumor necrosis factor (TNF)-related apoptosis-inducing ligand [2]) belongs to the TNF family and activates apoptosis in tumor cells. Three closely related receptors bind Apo2L: DR4 and DR5, which contain cytoplasmic death domains and signal apoptosis, and DcR1, a decoy receptor that lacks a cytoplasmic tail and inhibits Apo2L function [3-5]. By cross-hybridization with DcR1, we have identified a fourth Apo2L receptor, which contains a cytoplasmic region with a truncated death domain. We subsequently named this protein decoy receptor 2 (DcR2). The DcR2 gene mapped to human chromosome 8p21, as did the genes encoding DR4, DR5 and DcR1. A single DcR2 mRNA transcript showed a unique expression pattern in human tissues and was particularly abundant in fetal liver and adult testis. Upon overexpression, DcR2 did not activate apoptosis or nuclear factor-kappaB; however, it substantially reduced cellular sensitivity to Apo2L-induced apoptosis. These results suggest that DcR2 functions as an inhibitory Apo2L receptor.

展开

关键词：

Humans Hela Cells Chromosomes, Human, Pair 8 Membrane Glycoproteins Recombinant Fusion Proteins Receptors, Tumor Necrosis Factor Antigens, CD95 Base Sequence Sequence Homology, Amino Acid Apoptosis