摘要：

The present invention relates to the field of biotechnology and to recombinant DNA techniques and particularly to a process for the expression of the gene which codes for the protein of the envelope of the dengue viruses serotypes 2 and 4 in methylotrophic yeasts of the genus Pichia. The objective is to obtain an immunogene for human vaccination. For the dengue serotype 2 virus, we started from the genic sequence which codes for the protein E of the virus, from the strain A 15 isolated during the dengue fever epidemic and haemorrhagic dengue which occurred in Cuba in 1981. In the case of the serotype 4 dengue virus, we started from the genic sequence which codes for the protein E of the virus from the strain 814669 isolated during the dengue fever epidemy which occurred in Dominica in 1981.

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