Purification of the DNA polymerase of avian myeloblastosis virus
摘要:
DNA polymerase from avian myeloblastosis virus has been purified by a combination of column chromatography and gel filtration methods. The isolated enzyme sediments at approximately 6 S and consists of two subunits of molecular weights 110 000 and 69 000. It is free of RNA and DNA endonuclease activity. The enzyme possesses the RNA-, DNA-, and hybrid-directed polymerase activities found in the virion.
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关键词:
AMV avian myeloblastosis virus avian myeloblastosis virus BBOT 2,5-bis-2-(5- tert.-butylbenzoxazolyl)thiophene 2,5-bis-2-(5- tert.-butylbenzoxazolyl)thiophene
DOI:
10.1016/0005-2787(71)90773-8
被引量:
年份:
1971
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