Coordinate regulation of Bacillus subtilis peroxide stress genes by hydrogen peroxide and metal ions
摘要：
The Bacillus subtilis mrgA gene encodes an abundant DNA-binding protein that protects cells against the lethal effects of H2O2. Transcription of mrgA is induced by H2O2or by entry into stationary phase when manganese and iron levels are low. We have selected for strains derepressed for transcription of mrgA in the presence of Mn(II). The resulting cis-acting mutants define an operator site just upstream of the mrgA promoter. Similar sequences flank the promoters for the catalase gene, katA, and the heme biosynthesis operon, hemAXCDBL. Like mrgA, transcription of the katA and hem genes is repressed by Mn(II), which thereby potentiates the killing action of H2O2. We identified two classes of trans-acting mutants derepressed for mrgA transcription in the presence of Mn(II): some exhibit a coordinate derepression of MrgA, catalase, heme biosynthesis, and alkyl hydroperoxide reductase and are H2O2resistant, while others have reduced catalase activity and are H2O2sensitive. These data indicate that the peroxide stress response of B. subtilis is regulated by a repressor that senses both metal ion levels and H2O2.
展开
关键词：
Catalase Heme Manganese Iron Transcription Oxidative stress Escherichia-coli Salmonella-typhimurium Dna damage Cloning Sporulation Dismutation Expression Catalases Promoters
DOI：
10.1073/pnas.92.18.8190
被引量：
年份：
1995
国家科技图书文献中心
(权威机构)
掌桥科研
Semantic Scholar
万方医学
dx.doi.org
查看更多
Oxford Univ Press
NCBI
NCBI
(全网免费下载)
Europe PMC
(全网免费下载)
NCBI
JSTOR
Europe PMC
JBC
adsabs.harvard.edu
ResearchGate
(全网免费下载)
ResearchGate
EBSCO
onAcademic
mendeley.com
pnas.org
scienceopen.com
jb.asm.org
mysciencework.com
钛学术
core.ac.uk
pnas.org
(全网免费下载)
deepdyve.com
The National Academy of Sciences of the United States of America
(全网免费下载)
钛学术
(全网免费下载)
NCBI
(全网免费下载)
Europe PMC
(全网免费下载)
ResearchGate
(全网免费下载)
钛学术
(全网免费下载)
pnas.org
(全网免费下载)
The National Academy of Sciences of the United States of America
(全网免费下载)
通过文献互助平台发起求助，成功后即可免费获取论文全文。
相似文献
参考文献
引证文献
来源期刊
引用走势
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
