Crystal structures of human serum albumin complexed with monounsaturated and polyunsaturated fatty acids.
摘要：
The primary ligands of human serum albumin (HSA), an abundant plasma protein, are non-esterified fatty acids. In vivo, the majority of fatty acids associated with the protein are unsaturated. We present here the first high-resolution crystal structures of HSA complexed with two important unsaturated fatty acids, the monounsaturated oleic acid (C18:1) and the polyunsaturated arachidonic acid (C20:4). Both compounds are observed to occupy the seven binding sites distributed across the protein that are also bound by medium and long-chain saturated fatty acids. Although C18:1 fatty acid binds each site on HSA in a conformation almost identical with that of the corresponding saturated compound (C18:0), the presence of multiple cis double bonds in C20:4 induces distinct binding configurations at some sites. The observed restriction on binding configurations plausibly accounts for differences in the pattern of binding affinities for the primary sites between polyunsaturated fatty acids and their saturated or monounsaturated counterparts.
展开
关键词：
HSA human serum albumin human serum albumin C18:1 cis-9-octadecenoic acid (oleic acid cis-9-octadecenoic acid (oleic acid C18:2 cis-9,12-octadecadienoic acid (linoleic acid cis-9,12-octadecadienoic acid (linoleic acid C20:4 cis-5,8,11-14-eicosatetraenoic acid (arachidonic acid cis-5,8,11-14-eicosatetraenoic acid (arachidonic acid
DOI：
10.1006/jmbi.2000.5208
被引量：
年份：
2001
Elsevier
(全网免费下载)
Elsevier
万方医学
掌桥科研
dx.doi.org
查看更多
Ingenta
NCBI
NCBI
Europe PMC
EureKaMag.com
(全网免费下载)
ResearchGate
EBSCO
pdb.org
mendeley.com
onAcademic
jlr.org
rcsb.org
ajprenal.physiology.org
mysciencework.com
scienceopen.com
SAGE
deepdyve.com
readcube.com
scilit.net
www.socolar.com
pharmgkb.org
Elsevier
(全网免费下载)
EureKaMag.com
(全网免费下载)
通过文献互助平台发起求助，成功后即可免费获取论文全文。
相似文献
参考文献
引证文献
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
