Immunofluorescence microscopy and fluorescence in situ hybridization combined with CMEIAS and other image analysis tools for soil- and plant-associated microbial autecology
摘要：
This chapter describes the application of fluorescent molecular probes used with immunofluorescence microscopy (IFM) and fluorescence in situ hybridization (FISH) techniques for studies of microbial autecology, with an emphasis on soil and root-associated microbes. The procedures to prepare rabbit polyclonal antibodies to surface antigens of microbes, their conjugation to a fluorochrome, prepar...
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关键词：
antibodies antibody formation autecology fluorescence in situ hybridization image analysis immunization immunofluorescence metabolism microbial ecology microscopy
DOI：
10.1128/9781555815882.CH59
被引量：
年份：
2007
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