IMMUNOASSAY ON A PREBLOCKED SOLID SURFACE
申请(专利)号：
CA19892000685
申请日期：
1989-10-13
公开/公告号：
CA2000685C
公开/公告日期：
2001-02-20
申请(专利权)人：
BECTON, DICKINSON AND COMPANY
摘要：
A method far immunoassay of a viral antigen is performed on a membrane precoated with an inert protein. Nonimmunological capture of antigen takes place by absorption onto the coated membrane. Captured antigen binds to a tracer which includes a label conjugated to a specific antibody, the inert protein concomitantly inhibiting nonspecific binding of tracer. The label may be an enzyme which converts a substrate to a detectable product or converts a blocked inhibitor to an inhibitor whereby a second enzyme is inhibited from converting a substrate o a product. The invention includes a kit of materials for performing an assay in accordance with the method of the invention.
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