CELL BIOLOGY AND METABOLISM: Caspase-9, Bcl-XL, and Apaf-1 Form a Ternary Complex
摘要：
FIG. 1. Dominant negative caspase-9 (caspase-9-DN) blocked apoptosis in- duced by FADD, TRADD, and CED-4. A, a schematic presentation of Apaf-1. Apaf-1 contains an NH2-terminal CED-3- homologous region (amino acids 1- 89) that includes a CARD domain as indicated, a CED-4-homologous segment (amino acids 92- 412) and a COOH-terminal sequence that includes 12 WD-40 repeats as shown. The P-loop sequence for nucleotide binding and a putative Mg2ϩ binding site are also indicated. B, FADD-, TRADD-, and CED- 4-induced cell death is inhibited by caspase-9-DN. MCF7 cells were co-trans- fected with FADD, TRADD, or CED-4 to- gether with a -galactosidase-expressing reporter construct in the presence of a 3-fold excess of either vector or caspase-9- DN. Fifteen hours after transfection, cells were stained and examined as described (28). The data (mean Ϯ S.D.) represent the percentage of round, apoptotic cells as a function of total -galactosidase-positive cells (n ϭ 3). DN, dominant negative.
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