Control of Membrane Fusion by Divalent Cations, Phospholipid Head-Groups and Proteins

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作者：

Nejat Düzgüne，J Wilschut，D Papahadjopoulos

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摘要：

Membrane fusion is a vital process for many cellular activities such as exocytosis, endocytosis, formation of secondary lysosomes, membrane biosynthesis and cell division. During exocytosis secretory vesicles containing proteins or neurotransmitters fuse with the plasma membrane and release their contents into the extracellular space. Such fusion events have been observed by electron-microscopy in many cell types, for example during histamine release in mast cells (Lagunoff, 1973; Lawson et al ., 1977), insulin secretion in pancreatic B-cells (Orci et al ., 1977), mucocyst secretion in Tetrahymena (Satir et al ., 1973), chromaffin granule extrusion in the adrenal medulla (Douglas, 1968) and neurotransmitter release at the neuromuscular junction (Ceccarelli et al ., 1972; Heuser et al ., 1979). The involvement of Ca 2+ in exocytosis has been shown by microinjection of Ca 2+ into cells (Miledi, 1973; Kanno et al ., 1973), or by using Ca 2+ -ionophores (Foreman et al ., 1973; Cochrane and Douglas, 1974). Llinas and Nicholson (1975) have shown the increase in intracellular Ca 2+ concentration following electrical stimulation of the squid giant synapse, and Baker and Knight (1978) have demonstrated the dependence of catecholamine release from adrenal medullary cells on the free Ca 2+ -concentration. The site of action of Ca 2+ or the mechanisms which control membrane fusion during excytosis are not known, however.

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DOI：

10.1007/978-1-4684-7538-8_14

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年份：

1985