Plasma Membrane Aquaporins Are Involved in Winter Embolism Recovery in Walnut Tree
摘要：
Cultured HeLa cells were irradiated with a single acute dose of either gamma rays (40 Gy) or low-energy proton beams (20 Gy). (1)H magnetic resonance spectra of intact cells harvested at different times after irradiation and of the correspondent perchloric acid (PCA) extracts prepared at different times after irradiation were run. Selected signals from glutathione and lactate were examined with the aim of investigating effects of irradiation on antioxidative stores and on mitochondrial activity. An increase of signal intensity of glutathione (GSH) takes place at 15 and 24 hr after irradiation, while a decrease of its signal intensity, accompanied by an increase of that of free glutamate, starts appearing 48 hr after irradiation. Lactate signal increases 48 hr after irradiation. Signals from lipid catabolites were also examined to explore their sensitivity in predicting the response to radiotherapy. Intensity ratios of signals of glycerophosphorylcholine and choline to that of phosphorylcholine increase with time after irradiation. Irradiating cells with gamma rays or proton beams at half a dose produces effects comparable to the metabolic variations presented here. The present experiments allow more insight into the complex pattern of the changes of GSH by irradiation and indicate that magnetic resonance spectroscopy signals from GSH, glutamate, lactate, and lipid catabolites are affected by irradiation. Finally, these data represent a first indication that the relative biological efficiency for some metabolic damage of low-energy proton beams with respect to gamma rays can reach a value of 2.
展开
关键词：
DOI：
10.1104/pp.103.027797
被引量：
年份：
2003
academic.oup.com
doi.org
万方医学
掌桥科研
dx.doi.org
查看更多
Europe PMC
NCBI
(全网免费下载)
NCBI
Oxford Univ Press
JSTOR
AGRIS
ResearchGate
ProQuest
ResearchGate
(全网免费下载)
EBSCO
hal.univ-nantes.fr
plantphysiol.org
plantphysiology.org
(全网免费下载)
hal.univ-grenoble-alpes.fr
core.ac.uk
plantphysiology.org
publications.cirad.fr
hortsci.ashspublications.org
hal.archives-ouvertes.fr
hal.in2p3.fr
dumas.ccsd.cnrs.fr
hal.ird.fr
hal.univ-savoie.fr
pubfacts.com
deepdyve.com
hal.inserm.fr
hal.univ-brest.fr
plantphysiol.org
(全网免费下载)
amjbot.org
onAcademic
mysciencework.com
通过文献互助平台发起求助，成功后即可免费获取论文全文。
相似文献
参考文献
引证文献
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
