Strength of Signal through BCR Determines the Fate of Cycling B Cells by Regulating the Expression of the Bcl-2 Family of Survival Proteins

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摘要：

Cycling, splenic B cells were recultured with: (1) no stimulant to reflect poorly competitive clones; (2) soluble, whole anti-μ to reflect clones that bind soluble immune complexes; (3) soluble F(ab′) 2 anti-μ to reflect clones that bind soluble antigen; and (4) immobilized anti-μ to reflect clones that bind antigen presented by FDC. All four groups displayed similar levels of the death proteins Bax and Bcl-x S . In contrast, cycling B cells restimulated with either soluble F(ab′) 2 or immobilized anti-μ expressed heightened levels of the survival protein Bcl-x L , and only cells restimulated with immobilized anti-μ expressed the survival protein Bcl-2. Cycling B cells restimulated with either soluble F(ab′) 2 or immobilized anti-μ displayed a selective survival advantage over cycling B cells receiving no stimulus or soluble, whole anti-μ by both enhancing their responsiveness to CD40 ligand, a Th-cell-derived signal, and increasing the period that the cycling B cells remained responsive to this Th-cell-derived signal. The Th-cell-derived signal did not appreciably alter cycling B cell expression of Bcl-2 family members.

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Animals Rabbits Mice B-Lymphocytes Spodoptera Cells, Cultured Cell Line Membrane Glycoproteins CD40 Ligand Receptors, Antigen, B-Cell