Determination of microgram quantities of protein in the Bradford Coomassie brilliant blue assay is accomplished by measurement of absorbance at 590 nm. How...
Protein tyrosine phosphatases regulate signal transduction pathways involving tyrosine phosphorylation and have been implicated in the development of cance...
The second messenger hydrogen peroxide is required for optimal activation of numerous signal transduction pathways, particularly those mediated by protein ...
Zinc is an essential trace element required for enzymatic activity and for maintaining the conformation of many transcription factors; thus, zinc homeostas...
Thionein (T) has not been isolated previously from biological material. However, it is generated transiently in situ by removal of zinc from metallothionei...
Zinc plays both physiological and pathological roles in biology, making it of increasing interest. To date, intracellular free zinc has been measured in ce...
Epidermal growth factor stimulates a cyclic AMP-independent protein kinase associated with membrane vesicles derived from human epidermoid carcinoma cells ...
In spite of the paramount importance of zinc in biology.. dynamic aspects of cellular zinc metabolism remain poorly defined at the molecular level. Investi...
We developed genetically encoded fluorescence resonance energy transfer (FRET)-based sensors that display a large ratiometric change upon Zn(2+) binding, h...
The essentiality of zinc in humans was established in 1963. During the past 50 y, tremendous advances in both clinical and basic sciences of zinc metabolis...
Several pathways increase the concentrations of cellular free zinc(II) ions. Such fluctuations suggest that zinc(II) ions are signalling ions used for the ...
Several pathways increase the concentrations of cellular free zinc(II) ions. Such fluctuations suggest that zinc(II) ions are signalling ions used for the ...
Zinc exists in biological systems as bound and histochemically reactive free Zn 2+ in the nanomolar range. Zinc is required as either structural or cataly...
Rtr1 is a new phosphatase unrelated structurally to known such enzymes.Rtr1's N-terminal domain represents a novel fold for phosphatase function.Disruption...
ZnONPs at non-toxic concentrations increased the toxicity of CuNPs in vitro.ZnONPs of larger size provoked a stronger synergistic effect with CuNPs.The syn...
Fluorescent sensors are powerful tools for visualizing and quantifying molecules and ions in living cells. A variety of small molecule and genetically encoded sensors have been developed for studying intracellular Zn2+ homeostasis and si...
Zinc is an essential trace element that plays a vital role in many biological processes including growth and development, immunity, and metabolism. Recent ...
Mild dietary zinc deprivation in humans and rodents has little effect on blood plasma zinc levels, and yet cellular consequences of zinc depletion can be d...
