Completing the series of BVMOs involved in camphor metabolism of Pseudomonas putida NCIMB 10007 by identification of the two missing genes, their functional expression in E. coli, and biochemical characterization
摘要：
4,5,5-trimethylcyclopentenylacetyl-CoA monooxygenase (OTEMO) have not been entirely studied, since it was not possible to produce those enzymes in satisfactory amounts and purity. In this study, we were able to clone and recombinantly express both enzymes and subsequently use them as biocatalysts for various mono- and bicyclic ketones. Full conversion could be reached with both enzymes towards (±)--bicyclo[3.2.0]hept-2-en-6-one and with 3,6-DKCMO towards ()-camphor. Further OTEMO gave full conversion with norcamphor. OTEMO was found to have a pH optimum of 9 and a temperature optimum of 20°C and converted (±)--bicyclo[3.2.0]hept-2-en-6-one with a / value of 49.3mMs.
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DOI：
10.1007/s00253-011-3859-1
被引量：
年份：
2012
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