Three abundance classes in HeLa cell messenger RNA

Approximately 35,000 different poly(A)-containing RNA sequences are present in HeLa cell cytoplasm. The sequences are grouped in three distinct abundance c...

JO Bishop，JG Morton，M Rosbash，... - 《Nature》

Localization of the human α-globin structural gene to chromosome 16 in somatic cell hybrids by molecular hybridization assay

We have used 16 human × mouse somatic cell hybrids containing a variable number of human chromosomes to demonstrate that the human α-globin gene is on ch...

A Deisseroth，A Nienhuis，P Turner，... - 《Cell》

Enzymatic in vitro synthesis of globin genes

Full-length, single-stranded rabbit globin cDNA, synthesized by AMV reverse transcriptase, apparently contains a small double-stranded sequence (hairpin) at the 3鈥 terminus. This cDNA can serve as template-primer for E. coli DNA polymer...

A Efstratiadis，FC Kafatos，AM Maxam，... - 《Cell》

globin messenger-rna induction during erythroid differentiation of cultured leukemia

A cloned line of murine proerythroblastoid cells (T-3-Cl-2), transformed by Friend leukemia virus, undergoes changes associated with erythroid differentiation when treated with dimethylsulfoxide in culture. This line, which does not unde...

Jeffrey Ross，Yoji Ikawa，Philip Leder - 《Proceedings of the National Academy of Sciences》

Temperature‐sensitive dna polymerase from rous sarcoma virus mutants

The DNA polymerase ("reverse transcriptase") has been isolated from virions of two temperature-sensitive Rous sarcoma virus mutants (ts335 and ts337). Thes...

D Baltimore，IM Verma，S Drost，... - 《Cancer》

The synthesis of high yields of full-length reverse transcripts of globin mRNA

Conditions have been determined under which reverse transcriptase catalyzes the synthesis of high yields of full length complementary deoxyribonucleic acid (cDNA). These conditions depend not only on the concentration of deoxynucleoside ...

EY Friedman，M Rosbash - 《Nucl Acids Res》

Reverse transcriptase

Recognition of RNA tumor viruses as causative agents of malignant disease started with the observation (by Ellerman and Bang in 1908) that a filterable age...

A M Wu，R C Gallo - 《Crc Critical Reviews in Biochemistry》

Hemoglobin synthesis in somatic cell hybrids: globin gene expression in hybrids between mouse erythroleukemia and human marrow cells or fibroblasts.

Somatic cell hybrids were generated by fusion of mouse erythroleukemia cells either to mouse L cells (B82), human fibroblasts (W1-18 VA2), or human marrow ...

A Deisseroth，R Burk，D Picciano，... - 《Proceedings of the National Academy of Sciences of the United States of America》

Reverse Transcriptase

Reverse transcriptase (RT) is the key enzyme required for conversion of RNA to DNA. Cloning of Moloney murine leukemia virus (MMLV) RT has enable engineeri...

D Rifkind，GL Freeman - 《Molecular Biotechnology》

The Molecular Biology of the Thalassemia Syndromes

CRC Crit Rev Biochem. 1978;5(4):343-67. Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.; Review

Arthur，Bank，Francesco，... - 《Crc Critical Reviews in Biochemistry》