摘要：

Objective To study the inhibition of berberine( BBR) against ECV-304 apoptosis induced byStaphylococcus aureus(S. aureus). Methods ECV-304 cells were pre-treated with 128 μg /mL BBR for 2 h and thenS. aureus was added( 1: 100). The viability of cells was detected by MTT( 3-4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The morphological changes were observed by Hoechst 33258 staining. The protection of BBR for infected cells was detected by DNA Ladder. Results ECV-304 cells' viability were not obviously affected by berberine. ButS. aureus induced ECV-304 cells' viability could be significantly inhibited by pre-treatment of BBR(P 0. 05). BesidesS. aureus-induced ECV-304 apoptosis could be reduced,with significantly lessened apoptotic body and unobvious DNA degradation.Conclusion BBR could significantly inhibitS. aureus induced ECV-304 apoptosis.

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