Transcriptionally inactive oocyte-type 5S RNA genes of Xenopus laevis are complexed with TFIIIA in vitro.
摘要：
Anextract fromwholeoocytes ofXenopus laevis wasshowntotranscribe somatic-type 5SRNA genes -100-fold moreefficiently thanoocyte-type 5SRNAgenes. Thispreference wasatleast 10-fold greater thanthe preference seenuponmicroinjection of5SRNAgenes into oocyte nuclei oruponinvitro transcription inan oocyte nuclear extract. The- 100-fold transcriptional bias infavor ofthesomatic-type 5SRNAgenes observed invitro inthewholeoocyte extract wassimilar tothetranscriptional biasobserved indeveloping Xenopus embryos. We also showed that inthewholeoocyte extract, apromoter-binding protein required for5SRNA genetranscription, TFIHA,wasboundbothtotheactively transcribed somatic-type 5SRNAgenes andtothe largely inactive oocyte-type 5SRNA genes. Thesefindings suggest thatthemechanism forthedifferential expression of5SRNAgenes during Xenopus development doesnotinvolve differential binding ofTFIIIA to5S RNA genes. ThegenomeofthefrogXenopuslaevis contains two distinct families ofgenesfor5SRNA,thesmallest RNA component oftheribosome. Inthedeveloping oocyte ofthis
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DOI：
10.1128/MCB.7.10.3503
被引量：
年份：
1987
Semantic Scholar
万方医学
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掌桥科研
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