Raft formation and enlargement was investigated in liposomes and supported bilayers prepared from sphingomyelin (SM), cholesterol, and unsaturated phosphol...
Examines the role of the integral membrane P-type adenosine triphosphatase (ATPase) Drs2p in yeast late Golgi function. Effect of aspartic acid 560 mutatio...
The method developed previously for formation of unilamellar vesicles from mixed micelles of egg lecithin and octyl glucoside [Mimms, L. T., Zampighi, G., Nozaki, Y., Tanford, C., & Reynolds, J. A. (1981) Biochemistry 20, 833-840] has be...
Phospholipid and non-phospholipid vesicles are extensively studied as drug delivery systems to modify pharmacokinetics of drugs and to improve their action...
Thermodynamically stable vesicle (L α1) formation from glycolipid biosurfactant sponge phase (L 3) and its mechanism were investigated using a "natural" b...
We introduce the use of silicon (Si) as a substrate for the electroformation of giant phospholipid vesicles. By taking advantage of the tunability of silic...
Leek (Allium porrum) plasma membrane is enriched in phosphatidylserine (PS) by the vesicular pathway, in a way similar to that already observed in animal c...
This investigation presents a quantitative analysis of the asymmetric transmembrane lipid packing geometry in egg phosphatidylcholine vesicles. The analysi...
Single shelled lecithin vesicles of uniform size ( diameter = 300 A ̊) are prepared without sonication by solubilizing unsonicated lecithin dispersions ...
The molecular weight of the major glycoprotein from the human erythrocyte membrane is 29,000, of which 55% is carbohydrate and 45% is protein. The binding ...
SUMMARYTwo methods of freezing samples for freeze-fracture electron microscopy have been compared using X-ray diffraction and freeze-fracture results from ...
Bilayer membranes formed from lipids dissolved in decane were exposed to glycophorin, a sialoglycoprotein which had been extracted from human red cell memb...
The coat protein of fd bacteriophage has a short polypeptide chain of only 50 amino acid residues, containing a highly hydrophobic segment of 19 amino acid...
A variety of reconstitution procedures has been developed for the analysis of the biological activity of purified membrane proteins. In this paper we describe some new procedures. One is based on the action of two detergents, one ionic a...
A technique for the rapid production of large unilamellar vesicles by repeated extrusion under moderate pressures ( lb/ in 2) of multilamellar vesicles th...
Intercellular adhesion molecule-1 (ICAM-1) on the surface of cultured umbilical vein and saphenous vein endothelial cells was upregulated between 2.5- and ...
Affinity chromatography was used to identify a putative cell surface receptor for fibronectin. A large cell-attachment-promoting fibronectin fragment was u...
Mammalian cell receptors that promote entry of intracellular bacteria into nonphagocytic cells have not been identified. We show here that multiple members...
Although the majority of factor VII (FVII) circulates in the zymogen form, low levels of activated factor VII (FVIIa) have been postulated to exist in plas...
The lipid bilayer is one of the most eloquent and important self-assembled structures in nature. It not only provides a protective container for cells and ...
Gemcitabine (2',2'-difluorodeoxycytidine) is a novel pyrimidine nucleoside drug with clinical efficacy in several common epithelial cancers. We have propos...
Intercellular connexin channels (gap junction channels) have long been thought to mediate molecular signaling between cells, but the nature of the signalin...
The binding of factor VII and tissue factor produces a membrane-associated proteolytic complex which may be the primary biological initiator of coagulation...
Mammalian cell receptors that promote entry of intracellular bacteria into nonphagocytic cells have not been identified. We show here that multiple members of the integrin superfamily of cell adhesion receptors bind the Y. pseudotubercul...
