Establishment and characterization of a melanoma cell line from a xeroderma pigmentosum patient: activation of N-ras at a potential pyrimidine dimer site
摘要：
Patients suffering from the (XP) display an extreme sensitivity of their skin to sun (UV) exposure and predisposition to due to deficiencies in the excision pathway. Here we describe the establishment and characterization of the first cell line derived from an XP patient (belonging to complementation group C). The cell line designated XP44RO(Mel) has retained its tumorigenic and XP phenotype (UV sensitivity, reduced unscheduled DNA synthesis) and showed karyotypic abnormalities characteristic of . Transfection of XP44RO(Mel) DNA to NIH3T3 cells and oligonucleotide hybridization revealed that the N-oncogene was activated by an A.T to T.A or C.G transversion at the third position of codon 61. This mutation occurs at a dipyrimidine site. It is likely initiated by a UV-induced dimer and is of a type rarely observed in mammalian shuttle vector systems and endogenous genes after UV irradiation.
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DOI：
doi:10.1016/0304-3835(89)90066-9
被引量：
年份：
1989
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