摘要：

Background and purposes:Arachidonic acid cytochrome P450 (CYP) epoxygenases metabolize arachi-donic acid to produce Epoxyeicosatrienoic acids (EETs), also named endothelium-derived hyperpolarizing factors (EDHFs). It has demonstrated that EETs or EDHFs play an important role in the regulation of local microcirculation. Their regulatory effect on blood pressure, however, is unknown or controversial. The present study was designed to investigate the effects of delivery of arachidonic acid CYP epoxygenases gene on blood pressure in adultspontaneous hypertensive rats (SHRs) to insight whether the CYP epoxygenases or EDHFs play a role in blood pressure regulation. Method: Mammalian expression vector containing human cytochrome P450 epoxygenase CYP2J2 cDNA (1.5Kb), pcDNA-2J2 and pcDNA3.1 ware intravenously injected into adult spontaneously hypertensive rats, respectively, and then the blood pressure was measured twice a week using rat tail cuff sphygmomanometer until sacrifice at 3 or 4 weeks after the injection. The expression of CYP2J2 in various organs was detected by Western blotting. Result:The intravenous delivery of pcDNA.2J2 induced decrease in blood pressure in SHR compared with control group, and the hypoten-sive effect maintained 2-3 weeks. Western blotting showed detectable expression of human CYP2J2 in lung, live, heart and kidney detected by anti-human CYP2J2 antibodies. Conclusion: The present study showed that the delivery of human CYP2J2 gene induced the high CYP2J2 expression in the animal organs and caused a long-term reduction in blood pressure in SHRs simultaneously, which suggested that arachidonic acid cytochrome P450 epoxygenases involve in the regulation of the blood pressure in SHRs.

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