Methylated nucleotides block 5′-terminus of HeLa cell mRNA

The 5'-terminal oligonucleotides m7G(5')ppp(5')NmpNp and m7G(5')ppp(5')NmpNmpNp were isolated by DEAE-cellulose column chromatography after enzymatic digestion of 32P- or methyl-3H-labeled poly(A)" HeLa cell mRNA. The recovery of such ol...

CM Wei，A Gershowitz，B Moss - 《Cell》

Existence of methylated messenger RNA in mouse L cells

Messenger RNA of mouse L cells is methylated in both base and ribose moieties. On the average there are about 2.2 methyl groups per 1000 nucleotides in mRNA, a proportion which is about one-sixth that of mammalian ribosomal RNA. The vari...

RP Perry，DE Kelley - 《Cell》

Sequences containing methylated nucleotides at the 5′ termini of messenger RNAs: Possible implications for processing

Rottman F, Shatkin AJ, Perry RP.

F Rottman，AJ Shatkin，RP Perry - 《Cell》

In vitro synthesis of methylated messenger RNA by the virion-associated RNA polymerase of vesicular stomatitis virus

Purified vesicular stomatitis virus contains an enzyme activity that incorporates the methyl group of S-adenosyl-L-methionine into the messenger RNA produc...

Rhodes，D - 《Cell》

Translational capacity of deadenylated messenger RNA

In order to determine whether the functional capacity of polyadenylated messenger RNA is altered when the poly(A) segment is shortened as a consequence of mRNA aging, we compared the abilities of differentially labeled new and old mRNAs ...

E Bard - 《Cell》

Studies of nucleotide sequences in tobacco mosaic virus ribonucleic acid. IV. Use of aniline in stepwise degradation.

Aniline was found to be an effective catalyst for the release of phosphate from periodate-oxidized 5′-nucleotides at pH 5 and 25°. Aniline also eliminated the terminal residue of periodate-treated tobacco mosaic virus ribonucleic acid ...

A Steinschneider，H Fraenkel-Conrat - 《Biochemistry》

Transcription byInfectious Subviral Particles ofReovirus

Digestion of purified reovirus with chymotrypsin in the presence of 0.15 M NaCl converts virions to infectious subviral particles (SVP(i)). The SVP(i) have...

AJ Shatkinanda，J Lafiandra - 《Journal of Virology》

Mechanism of Action of the Mycotoxin Trichodermin, a 12,13-Epoxytrichothecene

Trichodermin is a member of a group of closely related compounds--the 12,13-epoxytrichothecenes--that form a medically and economically important class of ...

CM Wei，BS Hansen，VCS Mclaughlin - 《Proceedings of the National Academy of Sciences of the United States of America》

The 5′-Terminal Nucleotide Sequences of the Double-Stranded RNA of Human Reovirus

The 5′-terminal nucleotide sequences of human reovirus double-stranded RNA were determined after labeling the RNA with [32P]phosphate by polynucleotide ki...

KI Miura，K Watanabe，M Sugiura，... - 《Proceedings of the National Academy of Sciences of the United States of America》

5′-terminal nucleotide sequences of the double-stranded RNA of silkworm cytoplasmic polyhedrosis virus

The 5'-terminal nucleotide sequence of double-stranded RNA of silkworm cytoplasmic polyhedrosis virus was analyzed. In order to label the 5′-termini of th...

Kin-ichiro，Miura，and，... - 《Journal of Molecular Biology》