A detergent-free method for purifying caveolae membrane from tissue culture cells
摘要：
Current methods for purifying caveolae from tissue culture cells take advantage of the Triton X-100 insolubility of this membrane domain. To circumvent the use of detergents, we have developed a method that depends upon the unique buoyant density of caveolae membrane. The caveolae fractions that we obtain are highly enriched in caveolin. As a consequence we are able to identify caveolae-associated proteins that had previously gone undetected. Moreover, resident caveolae proteins that are soluble in Triton X-100 are retained during the isolation.
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关键词：
G proteins Epidermal growth factor receptor Folate receptor Gamma-subunits Proteins Receptor Transport Molecules Surface Pathway
DOI：
10.1073/pnas.92.22.10104
被引量：
年份：
1995
Taylor & Francis
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