Association of the protein tyrosine phosphatase PTP1C with the protein tyrosine kinase c-Src in human platelets

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作者：

H Falet，F Ramos-Morales，C Bachelot，S Fischer，F Rendu

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摘要：

Protein tyrosine phosphatase 1C (PTP1C), highly expressed in hematopoietic cells, is a soluble protein tyrosine phosphatase containing two Src homology 2 (SH2) domains at the N-terminus and two putative sites of tyrosine phosphorylation at the C-terminus. This paper reports that PTP1C and c-Src could be coimmunoprecipitated during thrombin-induced platelet activation. Moreover, association between the two signalling proteins occurred only after PTP1C had been tyrosine phosphorylated. In in vitro experiments, PTP1C bound to the SH2 domain of c-Src, suggesting that association between tyrosine phosphorylated PTP1C and c-Src was mediated by the SH2 domain of c-Src. Finally, in resting platelets, PTP1C was mainly found in the Nonidet P-40 soluble fraction whereas following thrombin-induced activation, around 17% of PTP1C was associated with the insoluble fraction.

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关键词：

Protein tyrosine phosphatase 1C c-Src SH2 domain Human platelet PTK, protein tyrosine kinase SH2 domain, Src homology 2 domain PTP, protein tyrosine phosphatase GST, glutathione S-transferase SDS-PAGE, SDS-polyacrylamide gel electrophoresis pTyr, phosphotyrosine