Ribonuclease P: unity and diversity in a tRNA processing ribozyme.

Ribonuclease P (RNase P) is the endoribonuclease that generates the mature 5'-ends of tRNA by removal of the 5'-leader elements of precursor-tRNAs. This en...

DN Frank，NR Pace - 《Annual Review of Biochemistry》

A yeast small nuclear RNA is required for normal processing of pre-ribosomal RNA.

In Saccharomyces cerevisiae, seven snRNAs (snR3, 4, 5, 8, 9, 10 and 17) are retained in the nucleus under conditions in which nucleoplasmic RNAs are lost, ...

D Tollervey - 《Embo Journal》

Yeast Sm-like proteins function in mRNA decapping and decay

One of the main mechanisms of messenger RNA degradation in eukaryotes occurs by deadenylation-dependent decapping which leads to 5'-to-3' decay. A family o...

S Tharun，W He，AE Mayes，... - 《Nature》

Identification of functional U1 snRNA-pre-mRNA complexes committed to spliceosome assembly and splicing

Although both U1 and U2 snRNPs have been implicated in the splicing process, their respective roles in the earliest stages of intron recognition and spliceosome assembly are uncertain. To address this issue, we developed a new strategy t...

B Seraphin，M Rosbash - 《Cell》

Identification and functional analysis of two U3 binding sites on yeast pre-ribosomal RNA.

It has long been known that U3 can be isolated hydrogen bonded to pre-ribosomal RNAs, but the sites of interaction are poorly characterized. Here we show t...

M Beltrame，D Tollervey - 《Embo Journal》

Sm and Sm-like proteins belong to a large family: identification of proteins of the U6 as well as the U1, U2, U4 and U5 snRNPs.

Several small nuclear RNAs (snRNAs), including the spliceosomal U1, U2, U4 and U5 snRNAs, are associated with Sm proteins. These eight small proteins form ...

B Séraphin - 《Embo Journal》

The Prp19p-Associated Complex in Spliceosome Activation

During spliceosome activation, a large structural rearrangement occurs that involves the release of two small nuclear RNAs, U1 and U4, and the addition of ...

SP Chan，DI Kao，WY Tsai，... - 《Science》

The POP1 gene encodes a protein component common to the RNase MRP and RNase P ribonucleoproteins.

Two forms of the yeast 5.8S rRNA are generated from a large precursor by distinct processing pathways. Cleavage at site A3 is required for synthesis of the...

Z Lygerou，P Mitchell，E Petfalski，... - 《Genes Dev》

Identification by mass spectrometry and functional analysis of novel proteins of the yeast [U4/U6·U5] tri-snRNP

The 25S [U4/U6·U5] tri-snRNP (small nuclear ribonucleoprotein) is a central unit of the nuclear pre-mRNA splicing machinery. The U4, U5 and U6 snRNAs unde...

A Gottschalk，G Neubauer，J Banroques，... - 《Embo Journal》

Maraia, R.J. & Intine, R.V. Recognition of nascent RNA by the human La antigen: conserved and diverged features of structure and function. Mol. Cell. Biol. 21, 367-379

La is a conserved RNA-binding phosphoprotein that inter- acts with a large variety of ligands. The most ubiquitous func- tion of La is association with newly synthesized RNA polymer- ase (Pol) III transcripts via their common UUU-OH 39 t...

RJ Maraia，RVA Intine - 《Molecular & Cellular Biology》