p27Kip1, a cyclin-Cdk inhibitor, links transforming growth factor-beta and contact inhibition to cell cycle arrest.
摘要：
Cell-cell contact and TGF-beta can arrest the cell cycle in G1. Mv1Lu mink epithelial cells arrested by either mechanism are incapable of assembling active complexes containing the G1 cyclin, cyclin E, and its catalytic subunit, Cdk2. These growth inhibitory signals block Cdk2 activation by raising the threshold level of cyclin E necessary to activate Cdk2. In arrested cells the threshold is set higher than physiological cyclin E levels and is determined by an inhibitor that binds to cyclin E-Cdk2 complexes. A 27-kD protein that binds to and prevents the activation of cyclin E-Cdk2 complexes can be purified from arrested cells but not from proliferating cells, using cyclin E-Cdk2 affinity chromatography. p27 is present in proliferating cells, but it is sequestered and unavailable to interact with cyclin E-Cdk2 complexes. Cyclin D2-Cdk4 complexes bind competitively to and down-regulate the activity of p27 and may thereby act in a pathway that reverses Cdk2 inhibition and enables G1 progression.
展开
关键词：
Animals Mink Cell Line Proteins Protein Kinases CDC2-CDC28 Kinases Cyclins Transforming Growth Factor beta Protein Kinase Inhibitors Cell Communication
DOI：
10.1101/gad.8.1.9
被引量：
年份：
1994
Taylor & Francis
BMJ
万方医学
Semantic Scholar
Oxford Univ Press
查看更多
Europe PMC
NCBI
AACR
JBC
ResearchGate
(全网免费下载)
ResearchGate
press.endocrine.org
mcb.asm.org
molpharm.aspetjournals.org
embor.embopress.org
SAGE
jn.nutrition.org
bloodjournal.org
scienceopen.com
molbiolcell.org
science-open.com
jcs.biologists.org
jacionline.org
pnas.org
ajpgi.physiology.org
mendeley.com
biochemj.org
jco.ascopubs.org
jcb.rupress.org
jasn.asnjournals.org
readcube.com
erc.endocrinology-journals.org
jimmunol.org
circres.ahajournals.org
genesdev.cshlp.org
transplantation-proceedings.org
eje-online.org
journalofsurgicalresearch.com
ResearchGate
(全网免费下载)
通过文献互助平台发起求助，成功后即可免费获取论文全文。
相似文献
参考文献
引证文献
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
