Methods and apparatus for measuring optical properties of biological and chemical substances

来自百度文库

喜欢 0

阅读量：

申请(专利)号：

US19960630054

申请日期：

1996-04-09

公开/公告号：

US5721435A

公开/公告日期：

1998-02-24

申请(专利权)人：

HEWLETT PACKARD COMPANY

发明人：

MA Troll

展开

被引量：

231

摘要：

An optical measurement system includes a test surface having disposed thereon a plurality reference markings and a plurality of test spots containing chemical and biological substances. Molecules of interest contained in the chemical and biological substances are tagged with optically detectable labels. A light source generates a beam of light which is then directed by a deflecting sub-system across the test surface, thereby individually impinging the test spots and the reference markings. Optical signals resulting from the impingement are detected by a detection means, which records such detection and the time of the detection. The reference markings have optically unique signatures to distinguish the markings from the optical signatures of the signals emitted from the chemical and biological substances in the test spots. The reference markings are spaced apart at known distances and serve to provide a constant calibration of the scan speed of the beam of light. In one embodiment, a Winston concentrator directs the light emanating from the test surface to the detector. In another embodiment, an off-axis parabolic mirror arrangement directs the light to the detector.

展开