摘要：

The enantiomeric resolution of clenbuterol was achieved on two Pirkle-type chiral stationary phases( α-Burke- 2 and Pirkle- 1J) by high performance liquid chromatography. Factors affecting the enantioresolution,such as the type and concentration of buffer solution,the type and content of organic modifier and temperature were investigated. The optimal separation conditions were as follows: dichloromethane-ethanol( 19: 1,V/V) containing 5 mmol/L ammonium acetate as the mobile phase,at a flow rate of 2. 0 mL/min,column temperatureof 20 ℃. Under the above conditions,the enantiomers of clenbuterol were successfully separated on α-Burke- 2 and Pirkle- 1J chiral stationary pahses with the resolution factors of 1. 85 and 0. 64,respectively. The effective π- π donor-acceptor interaction and hydrogen bonding interaction between the two Pirkle-type chiral stationary phases and clenbuterol were expected to be responsible for the chiral recognition.The method is simple and rapid for the quality control and stereoselective pharmacokinetics study of clenbuterol.

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