Separation and Quantitative Determination of Clenbuterol Enantiomers by Capillary Electrophoresis
摘要：
Capillary zone electrophoresis(CZE) was used for the enantiomeric separation of racemic clenbuterol with an uncoated capillary and hydroxypropyl-β-cyclodextrin(HP-β-CD) as chiral selector.The type and concentration of β-CDs,pH and concentration of buffer,separation voltage,and operation temperature were studied and optimized,respectively.By using the concentration of 30 mmol/L HP-β-CD as chiral selector,50 mmol/L phosphate solution(pH 2.5) as running buffer,separation voltage of 24 kV,and operation temperature of 20℃,the baselines separation could be obtained with resolution of 6.78.The binding constants and thermodynamic parameters were evaluated for clenbuterol enantiomers to HP-β-CD.It was found that the chiral matching of HP-β-CD to clenbuterol enantiomers were related to the equilibrium constants of binding complexes and selectivity factor(α).The quantitative ability of CZE including linearity and precision are also investigated.The linearity coefficient of(-)-R-clunbuterol and(+)-S-clenbuterol were all above 0.998,and both RSD were all below 2.2%.
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DOI：
10.1016/S1872-2040(07)60090-5
被引量：
年份：
2007
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