Mechanism of antigen-driven selection in germinal centres

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148

作者：

YJ Liu，DE Joshua，GT Williams，CA Smith，J Gordon，ICM Maclennan

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摘要：

The high affinity of antibodies produced during responses to T-cell-dependent antigens is associated with somatic mutation in the variable region of the immunoglobulin. Indirect evidence indicates that: (1) this arises by a process of hypermutation, acting selectively on rearranged immunoglobulin variable-region genes, which is activated in centroblasts within germinal centres; and (2) centrocytes, the progeny of centroblasts, undergo selection on the basis of their ability to receive a positive signal from antigen. We have now performed experiments analysing this selection process, and found that, on culture, centrocytes isolated from human tonsil kill themselves within a few hours by apoptosis. This is not a feature of other tonsillar B cells. Centrocytes can be prevented from entering apoptosis if they are activated both through their receptors for antigen and a surface glycoprotein recognized by CD40 antibodies.

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关键词：

Humans B-Lymphocytes Cells, Cultured Antibodies Culture Techniques Phenotype Cell Survival Cytotoxicity, Immunologic Antigens, CD Antigens, Surface