Chapter 16 Immunofluorescence Microscopy
摘要：
This chapter provides a practical guide to immunofluorescence microscopy of Caenorhabditis elegans. In this method, fixed tissue is stained with a fluorescently labeled antibody, and visualized with the light microscope. The antibody ensures that staining is limited to the location of the antigen, and the microscope provides a magnified image of the fluorescent area. Immunofluorescence microscopy is an especially powerful tool for studies of C. elegans. Immunofluorescence staining can provide essential information for the analysis of mutant phenotypes. Mutations that alter developmental programs or the expression of particular proteins can be examined in situ and described at the level of individual cells or cell lineages. Immunofluorescence microscopy can be used to detect the expression of transgenes in C. elegans. Specific epitope tags can be incorporated into a transgene, such that the epitope-tagged protein can be detected with a specific antibody that does not react with the endogenous protein. Epitope tagging is particularly useful for cases in which a null mutation in the endogenous gene is either not available or desirable in the transgenic animal.
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DOI：
10.1016/S0091-679X(08)61396-5
被引量：
年份：
1995
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