The CD4 receptor is complexed in detergent lysates to a protein-tyrosine kinase (pp58) from human T lymphocytes
摘要：
The CD4 (T4) antigen is a cell-surface glycoprotein that is expressed predominantly on the surface of helper T cells and has been implicated in the regulation of T-cell activation and in the associative recognition of class II antigens of the major histocompatibility complex. In addition, the CD4 antigen appears to serve as a receptor for the human immunodeficiency virus (HIV). An important question has been whether the CD4 receptor is linked to an intracellular mediator that could regulate the activation of the CD4+ subset. In this paper, we provide preliminary evidence that the CD4 receptor is complexed in detergent lysates to a protein-tyrosine kinase (PTK) of 55-60 kDa, which is expressed specifically in T cells. The PTK is the human analogue of the murine pp56LSTRA (pp56lck) and has significant homology with c-src, c-yes, and other members of the src family. The identification of the PTK associated with CD4 receptor was made by use of an antiserum to a synthetic peptide that was deduced from the DNA sequence of PTK. Two-dimensional nonequilibrium pH gradient gel electrophoresis/NaDodSO4/PAGE revealed the kinase to focus as a heterogeneous collection of spots in the pH range of 4.0-5.0. Furthermore, in vitro phosphorylation revealed the phosphorylation of two additional polypeptides at 40 and 80 kDa, in addition to the autophosphorylation of the PTK at 55-60 kDa. The potential importance of the association between the CD4 receptor and the PTK of T cells is discussed in relation to T-cell activation and HIV infectivity.
展开
关键词：
DOI：
10.1073/pnas.85.14.5190
被引量：
年份：
1988
Semantic Scholar
掌桥科研
万方医学
dx.doi.org
NCBI
查看更多
NCBI
Europe PMC
(全网免费下载)
JSTOR
Pubmed Central
(全网免费下载)
Europe PMC
NCBI
(全网免费下载)
JBC
AACR
adsabs.harvard.edu
ResearchGate
EBSCO
jimmunol.org
readcube.com
onAcademic
biochemj.org
jvi.asm.org
core.ac.uk
The National Academy of Sciences of the United States of America
(全网免费下载)
pubfacts.com
scienceopen.com
mysciencework.com
molbiolcell.org
pnas.org
mendeley.com
jleukbio.org
pnas.org
(全网免费下载)
jem.rupress.org
NCBI
(全网免费下载)
Europe PMC
(全网免费下载)
Pubmed Central
(全网免费下载)
pnas.org
(全网免费下载)
The National Academy of Sciences of the United States of America
(全网免费下载)
通过文献互助平台发起求助，成功后即可免费获取论文全文。
相似文献
参考文献
引证文献
引用走势
站内活动
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
