In vitro transcription and processing of a yeast tRNA gene containing an intervening sequence
摘要：
A gene for Saccharomyces cerevisiae tRNA Trp has been sequenced which contains an intervening sequence of 34 bp (H.S. Kang and J. Abelson, unpublished results). The mutant yeast strain ts-136 accumulates a precursor to tRNA Trp which contains mature ends and is colinear with the tRNA Trp gene. A nuclear extract from Xenopus oocytes is capable of supporting transcription of the tRNA Trp gene contained on plasmid pBR313. The products are precursor tRNAs which contain the intervening RNA sequence. The Xenopus extract accurately splices the precursor transcript to mature-sized tRNA Trp.
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关键词：
Animals Xenopus Oocytes Cell-Free System Cell Nucleus Saccharomyces cerevisiae Transcription, Genetic Nucleic Acid Precursors RNA, Transfer Female
DOI：
10.1016/0092-8674(79)90166-1
被引量：
年份：
1979
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