Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized film

This paper reports that autoradiographic sensitivity can be greatly increased for isotopes which emit γ-rays (e.g., I) or high energy β particles (e.g., ...

Ronald，A.，Laskey，... - 《Febs Letters》

Chromatography of 32P‐Labelled Oligonucleotides on Thin Layers of DEAE‐Cellulose

A two-dimensional fractionation procedure has been developed for separating radioactively-labelled oligonucleotides of up to 50 residues long, using uniformly 32 P-labelled 5S RNA of Escherichia coli as a model compound. The method us...

GG Brownlee，F Sanger - 《Eur J Biochem》

Injected nuclei in frog oocytes: fate, enlargement, and chromatin dispersal.

A method is described by which nuclei associated with some cytoplasm can be rapidly prepared from a suspension of cells. The method involves the use of lys...

JB Gurdon - 《Journal of Embryology & Experimental Morphology》

New rapid gel sequencing method for RNA

METHODS for sequencing nucleic acids have improved significantly in the last few years due to the development of rapid 'read-off' gel methods. The first me...

A Simoncsits，GG Brownlee，RS Brown，... - 《Nature》

Nucleotide sequence of Xenopus borealis oocyte 5S DNA: comparison of sequences that flank several related eucaryotic genes.

Genomic Xenopus borealis oocyte-specific 5S DNA (Xbo) contains clusters of 5S rRNA genes. The number of genes varies among clusters, and the distance betwe...

LJ Korn，DD Brown - 《Cell》

Coupled transcription-Translation of DNA injected into Xenopus oocytes

A previous report from this laboratory showed that purified DNAs are transcribed after injection into the nucleus of Xenopus laevis oocytes (Mertz and Gurd...

EMD Robertis，JE Mertz - 《Cell》

Molecular characterisation of the tyrosine tRNA genes of yeast

MANY of the tRNA genes of yeast are observable genetically as nonsense suppressors 1 . In particular, the set of unlinked suppressor loci SUP2 to SUP8 an...

OLSON, MAYNARD V，MONTGOMERY, DONNA L，HOPPER, ANITA K，... - 《Nature》

Transcription and processing of intervening sequences in yeast tRNA genes.

Genes for yeast tRNA Tyr and tRNA Phe have been sequenced ( Goodman, Olson and Hall, 1977; Valenzuela et al., 1978) which contain additional nucleotides (i...

G Knapp，JS Beckmann，PF Johnson，... - 《Cell》

Purified DNAs are transcribed after microinjection into Xenopus oocytes

The possibility of using DNA-injected Xenopus laevis oocytes and eggs for studying the control of transcription in eukaryotes has been investigated. When p...

JE Mertz，JB Gurdon - 《Proceedings of the National Academy of Sciences of the United States of America》

Structure and processing of yeast precursor tRNAs containing intervening sequences

We have isolated a precursor of yeast tRNATyr and shown that it contains an intervening sequence identical to that found in the gene for tRNATyr. The confo...

 - 《Nature》