Self-splicing RNA: autoexcision and autocyclization of the ribosomal RNA intervening sequence of Tetrahymena.
摘要：
In the macronuclear rRNA genes of Tetrahymena thermophila, a 413 bp intervening sequence (IVS) interrupts the 26S rRNA-coding region. A restriction fragment of the rDNA containing the IVS and portions of the adjacent rRNA sequences (exons) was inserted downstream from the lac UV5 promoter in a recombinant plasmid. Transcription of this template by purified Escherichia coli RNA polymerase in vitro produced a shortened version of the prerRNA, which was then deproteinized. When incubated with monovalent and divalent cations and a guanosine factor, this RNA underwent splicing. The reactions that were characterized included the precise excision of the IVS, attachment of guanosine to the 5′ end of the IVS, covalent cyclization of the IVS and ligation of the exons. We conclude that splicing activity is intrinsic to the structure of the RNA, and that enzymes, small nuclear RNAs and folding of the pre-rRNA into an RNP are unnecessary for these reactions. We propose that the IVS portion of the RNA has several enzyme-like properties that enable it to break and reform phosphodiester bonds. The finding of autocatalytic rearrangements of RNA molecules has implications for the mechanism and the evolution of other reactions that involve RNA.
展开
关键词：
Animals DNA Restriction Enzymes DNA Transposable Elements RNA, Ribosomal Nucleic Acid Hybridization Base Sequence Lac Operon Plasmids Transcription, Genetic Genes
DOI：
10.1016/0092-8674(82)90414-7
被引量：
年份：
1982
Elsevier
Wiley
Semantic Scholar
(全网免费下载)
Semantic Scholar
万方医学
查看更多
dx.doi.org
BioOne
BioMed Central
Cell Press
(全网免费下载)
NCBI
NCBI
Cell Press
Europe PMC
ResearchGate
(全网免费下载)
ResearchGate
EBSCO
scienceopen.com
mendeley.com
dosequis.colorado.edu
(全网免费下载)
science-open.com
pubpeer.com
invenzone.com
scripts.iucr.org
genome.cshlp.org
www2.pitt.edu
pubfacts.com
mysciencework.com
tamu.edu
(全网免费下载)
tamuweb.tamu.edu
(全网免费下载)
Semantic Scholar
(全网免费下载)
Cell Press
(全网免费下载)
ResearchGate
(全网免费下载)
dosequis.colorado.edu
(全网免费下载)
tamu.edu
(全网免费下载)
tamuweb.tamu.edu
(全网免费下载)
通过文献互助平台发起求助，成功后即可免费获取论文全文。
相似文献
参考文献
引证文献
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
